Temporary

Part:BBa_J70559:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-02-17)

N-terminal fusion test: R0010:J70558 or R0010:J70550:E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 992
    Illegal AgeI site found at 1104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1940


Design Notes

will add B0034 and a six his tag in here later with the oligos


Source

after cutting with BsaXI add these inserts

forward1 (J70559-f1): TTCACACATACTAGAGAAAGAGGAGAAATACTAGATGCATCATC

forward2 (J70559-f2): ATCATCATCACAGCAGCGGCcgta

reverse1 (J70559-r1): CTCTAGTATGTGTGAAatt

reverse2 (J70559-r2): gGCCGCTGCTGTGATGATGATGATGATGCATCTAGTATTTCTCCTCTTT

References